With ''A. m. mellifera'' bees, it can be difficult to establish two-queen bee hives with a separating queen excluder. Usually, the other queen is killed and only one queen is accepted per colony. Then again, this behavior can also be an advantage when the old queens are changed to new ones.
Searching for the queen:
* Work with care and keep in mind that some of the queens can hide outstandingly well.
* The queen usually goes to the empty built frames, which have been recently given to the hive and are also occupied by worker bees.